Development of Rickettsia Specific Nested PCR Method Based on groEL Gene Sequences
Journal of Bacteriology and Virology
;
: 301-306, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-128193
ABSTRACT
To detect Rickettsia, we have developed a nested PCR method amplifying the groEL gene. Rickettsia strains were successfully amplified by this PCR method but the microorganisms causing other febrile diseases, such as Orientia tsutsugamushi, Coxiella burnetii, Ehrlichia sennetsu, Borrelia burgdorferi sensu lato, Borrelia hermsii, and Leptospira interrogans were not amplified. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Sixteen Haemaphysalis longicornis ticks were positive by this PCR assay. These results suggest that the new nested PCR method might be sensitive and useful for discrimination between Rickettsia and other febrile disease-causing microorganisms.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rickettsia
/
Orientia tsutsugamushi
/
Garrapatas
/
Borrelia
/
ADN
/
Grupo Borrelia Burgdorferi
/
Reacción en Cadena de la Polimerasa
/
Coxiella burnetii
/
Neorickettsia sennetsu
/
Discriminación en Psicología
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS