Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR
Intestinal Research
;
: 128-134, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-128540
ABSTRACT
BACKGROUND/AIMS:
This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR).METHODS:
Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR.RESULTS:
Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa.CONCLUSIONS:
Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunohistoquímica
/
Enfermedades Inflamatorias del Intestino
/
Colitis Ulcerosa
/
Enfermedad de Crohn
/
Reacción en Cadena de la Polimerasa
/
Colitis
/
Colon
/
Ciclooxigenasa 2
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Inflamación
Límite:
Humanos
Idioma:
Coreano
Revista:
Intestinal Research
Año:
2008
Tipo del documento:
Artículo
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