Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 51-57, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-128608
ABSTRACT
OBJECTIVE:
This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.METHODS:
Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 microm.RESULTS:
In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.CONCLUSION:
These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Zona Pelúcida
/
Blastocisto
/
Estructuras Embrionarias
/
Herpes Zóster
/
Mórula
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Clinical and Experimental Reproductive Medicine
Año:
2015
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS