Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column / 한국실험동물학회지
Laboratory Animal Research
;
: 139-144, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-129626
ABSTRACT
Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sefarosa
/
Proteínas
/
Cromatografía
/
Desoxirribonucleasas
/
Haemonchus
/
Intestinos
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Laboratory Animal Research
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS