Alum Directly Modulates Murine B Lymphocytes to Produce IgG1 Isotype
Immune Network
;
: 10-15, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-13003
ABSTRACT
Aluminum hydroxide (alum) is the most widely used adjuvant in human vaccines. Nevertheless, it is virtually unknown whether alum acts on B cells. In the present study, we explored the direct effect of alum on Ig expression by murine B cells in vitro. LPS-activated mouse spleen B cells were cultured with alum, and the level of isotype-specific Ig secretion, IgG1 secreting cell numbers, and Ig germ-line transcripts (GLT) were measured using ELISA, ELISPOT, and RT-PCR, respectively. Alum consistently enhanced total IgG1 production, numbers of IgG1 secreting cells, and GLTgamma1 expression. These results demonstrate that alum can directly cause IgG1 isotype switching leading to IgG1 production.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bazo
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Vacunas
/
Linfocitos B
/
Recuento de Células
/
Cambio de Clase de Inmunoglobulina
/
Compuestos de Alumbre
/
Hidróxido de Aluminio
/
Ensayo de Immunospot Ligado a Enzimas
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Immune Network
Año:
2013
Tipo del documento:
Artículo
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