Further Studies on Effects of Some Local Anesthetics on Calcium Binding to Lipid-extracted RBC Membrane Fragments: Effect of Modification of Carboxyl Group with Carbodiimide
Yonsei Medical Journal
;
: 1-8, 1975.
Artículo
en Inglés
| WPRIM
| ID: wpr-130718
ABSTRACT
The interaction of calcium and local anesthetics was investigated with the lipid extracted human RBC membrane fragments treated with carbodiimide in order to titrate carboxyl groups. A water soluble carbodiimide [1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide methotoluene-p-sulfonate], referred to as a carbodiimide reagent, and glycine methylester were used for this purpose. About 76% of carboxyl groups of the fragments were modified at a concentration of 0.05M carbodiimide reagent. The interaction of calcium and local anesthetics such as procaine and lidocaine with these fragments still showed typical competition. However, when the calcium binding was decreased to 8% at a higher concentration of carbodiimide reagent (0.08M), the local anesthetics still inhibited the calcium binding, but were not competitive in nature. In other words, if concentrations of the carbodiimide reagent were raised, the degree of inhibition by the local anesthetics was gradually decreased and was not competitive in nature. Finally, no inhibition was demonstrated when the concentration of the reagent was 0.1 to 0.4M. The above findings, seem to suggest that local anesthetics such as procaine and lidocaine interact with carboxyl groups, in addition to phosphodiester groups of phospholipids as previously reported, and inhibited competitively calcium binding to carboxyl groups of the membrane fragments.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Unión Proteica
/
Técnicas In Vitro
/
Carbodiimidas
/
Membrana Celular
/
Calcio
/
Eritrocitos
/
Anestésicos Locales
Límite:
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
1975
Tipo del documento:
Artículo
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