Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Journal of Veterinary Science
;
: 59-65, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-13091
ABSTRACT
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Virales
/
Pruebas de Neutralización
/
Vacunas Virales
/
Western Blotting
/
Inmunización
/
Organismos Libres de Patógenos Específicos
/
Herpesvirus Humano 1
/
Herpesvirus Bovino 1
/
Inmunidad Humoral
/
Vectores Genéticos
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2012
Tipo del documento:
Artículo
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