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Effects of human growth hormone on gonadotropin-releasing hormone neurons in mice / 소아과
Korean Journal of Pediatrics ; : 845-851, 2010.
Artículo en Inglés | WPRIM | ID: wpr-130959
ABSTRACT

PURPOSE:

Recombinant human growth hormone (rhGH) has been widely used to treat short stature. However, there are some concerns that growth hormone treatment may induce skeletal maturation and early onset of puberty. In this study, we investigated whether rhGH can directly affect the neuronal activities of of gonadotropin-releasing hormone (GnRH).

METHODS:

We performed brain slice gramicidin-perforated current clamp recording to examine the direct membrane effects of rhGH on GnRH neurons, and a whole-cell voltage-clamp recording to examine the effects of rhGH on spontaneous postsynaptic events and holding currents in immature (postnatal days 13-21) and adult (postnatal days 42-73) mice.

RESULTS:

In immature mice, all 5 GnRH neurons recorded in gramicidin-perforated current clamp mode showed no membrane potential changes on application of rhGH (0.4, 1 microgram/mL). In adult GnRH neurons, 7 (78%) of 9 neurons tested showed no response to rhGH (0.2-1 microgram/mL) and 2 neurons showed slight depolarization. In 9 (90%) of 10 immature neurons tested, rhGH did not induce any membrane holding current changes or spontaneous postsynaptic currents (sPSCs). There was no change in sPSCs and holding current in 4 of 5 adult GnRH neurons.

CONCLUSION:

These findings demonstrate that rhGH does not directly affect the GnRH neuronal activities in our experimental model.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Encéfalo / Hormona del Crecimiento / Hormona Liberadora de Gonadotropina / Pubertad / Hormona de Crecimiento Humana / Potenciales Sinápticos / Potenciales de la Membrana / Membranas / Modelos Teóricos / Neuronas Tipo de estudio: Estudio pronóstico Límite: Animales / Humanos Idioma: Inglés Revista: Korean Journal of Pediatrics Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Encéfalo / Hormona del Crecimiento / Hormona Liberadora de Gonadotropina / Pubertad / Hormona de Crecimiento Humana / Potenciales Sinápticos / Potenciales de la Membrana / Membranas / Modelos Teóricos / Neuronas Tipo de estudio: Estudio pronóstico Límite: Animales / Humanos Idioma: Inglés Revista: Korean Journal of Pediatrics Año: 2010 Tipo del documento: Artículo