The upstream sequence of Mycobacterium leprae 18-kDa gene confers transcription repression activity in orientation-independent manner
Experimental & Molecular Medicine
; : 510-514, 2004.
Article
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| ID: wpr-13641
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WPRO
ABSTRACT
In order to understand the role of the upstream region of the Mycobacterium leprae 18-kDa gene on the gene regulation, the region was divided into two at the -50 position from the first start codon of the gene and their effect on transcription was examined by using a LacZ transcriptional reporter gene assay. The presence of each of these two regions conferred transcription repression not only on its cognate M. lepraerae 18-kDa gene promoter, but also on a heterologous promoter such as the Mycobacterium bovis BCG hsp65 gene promoter. Moreover, it was found that these regions could confer transcription repression activity in both cases in an orientation-independent manner. Thus, these results indicate that the upstream region of the M. leprae 18-kDa gene harbors transcription repression responsive element(s) acting as an operator and can be further divided into two separately functional regions, suggesting a bipartite structure of the element(s). The identification of transcription repression activity of the upstream region in the M. leprae 18-kDa gene will contribute greatly for the understanding of the 18-kDa gene regulation mechanism, and provide also useful information for the manipulation of mycobacterium gene expression.
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Asunto principal:
Proteínas Bacterianas
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Transcripción Genética
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Regulación hacia Abajo
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Regulación Bacteriana de la Expresión Génica
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Elementos de Respuesta
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Mycobacterium leprae
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
2004
Tipo del documento:
Article