ICAM-1/LFA-1 interaction contributes to the induction of endothelial cell-cell separation: implication for enhanced leukocyte diapedesis
Experimental & Molecular Medicine
;
: 341-348, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-136585
ABSTRACT
The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-alpha induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules-1 (ICAM-1)/leukocyte function-associated antigen-1 interaction, as demonstrated by the following (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Movimiento Celular
/
Células Cultivadas
/
Antígeno-1 Asociado a Función de Linfocito
/
Técnica del Anticuerpo Fluorescente
/
Molécula 1 de Adhesión Intercelular
/
Células Endoteliales
/
Citometría de Flujo
/
Leucocitos
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2009
Tipo del documento:
Artículo
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