Polyphenol (-)-epigallocatechin gallate targeting myocardial reperfusion limits infarct size and improves cardiac function / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 169-175, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-138716
ABSTRACT
BACKGROUND:
This experiment was performed to determine the effect of polyphenolic (-)-epigallocatechin (EGCG), the most abundant catechin of green tea, given at reperfusion period.METHODS:
Isolated rat hearts were subjected to 30 min of regional ischemia and 2 h of reperfusion. Green tea extract (GT) was perfused with the following concentrations; 0, 0.5, and 1 micrometer (GT-O, GT-0.5, and GT-1, respectively). In a next experiment, hearts were assigned randomly to one of the following groups; Control, EGCG-1 (1 micrometer of EGCG), and EGCG-10 (10 micrometer of EGCG). GT and EGCG were perfused for a period of 5 min before and 30 min after reperfusion. For comparison of cardioprotection among groups, morphometric measurement was performed by 2,3,5-triphenyltetrazolium chloride staning.RESULTS:
GT 1 micrometer (10.3 +/- 2.1%, P < 0.05) significantly reduced infarct volume as a percentage of ischemic volume compared to untreated hearts (27.4 +/- 1.1%). EGCG 10 micrometer (13.2 +/- 4.0%) significantly reduced myocardial infarction compared to control hearts (27.2 +/- 1.4%, P = 0.002). After 2 h of reperfusion, cardiodynamic variables, including left ventricular developed pressure, rate-pressure produce, +dP/dt(max), and -dP/dt(min) were significantly improved by 10 micrometer of EGCG compared to control hearts (P = 0.01, 0.016, 0.009, and 0.019, respectively).CONCLUSIONS:
EGCG treatment at an early reperfusion period reduces myocardial infarction and improves cardiodynamics in isolated rat hearts.
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Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Té
/
Sales de Tetrazolio
/
Reperfusión Miocárdica
/
Reperfusión
/
Daño por Reperfusión
/
Catequina
/
Corazón
/
Isquemia
/
Infarto del Miocardio
/
Miocardio
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Journal of Anesthesiology
Año:
2010
Tipo del documento:
Artículo
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