Characterization of Insect Cells Transformed with Autographa calfornica Nuclear Polyhedrosis Virus IE1 Gene
Journal of the Korean Society of Virology
;
: 137-144, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-142027
ABSTRACT
Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa calfornica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Expresión Génica
/
Baculoviridae
/
Nucleopoliedrovirus
/
Células Sf9
/
Insectos
Idioma:
Coreano
Revista:
Journal of the Korean Society of Virology
Año:
1999
Tipo del documento:
Artículo
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