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Evaluation of the DG Gel System using the Microtube Column Agglutination Technique for Antibody Screening and Identification / 대한수혈학회지
Korean Journal of Blood Transfusion ; : 32-38, 2007.
Artículo en Coreano | WPRIM | ID: wpr-14267
ABSTRACT

BACKGROUND:

For the antibody screening test, the classical LISS tube indirect antiglobulin test has been replaced by the microtube column agglutination system in Korea. This system was first created in 1990 by Lapierre and it is distributed through DiaMed (DiaMed Ag, Cresssier, Morat, Switzerland) around the world. Similar systems, such as Ortho BioVue, have been developed and competed after that. We evaluated a newly developed microtube column agglutination system, DG Gel (Diagnostic Grifols, Barcelona, Spain), and we compare it with the other established systems.

METHODS:

In a comparative study, a total of 126 samples, including 76 antibody screening positive samples and 50 negative samples, were tested in parallel by the LISS/Coombs card (DiaMed Ag, Cresssier, Morat, Switzerland) and the DG Gel microtube column agglutination system. The positive samples that were proved by the LISS/Coombs card and the DG Gel system were identified by the ID-Dia panel (DiaMed Ag, Cresssier, Morat, Switzerland) and Identisera Diana (Diagnostic Grifols, Barcelona, Spain). Discrepant samples were rechecked with I, II and III cells that were supplied by the panel of the Korea Red Cross Blood Center.

RESULTS:

Among the 126 samples, the DG Gel antibody screening system showed 98.7% (75/76) sensitivity and 100% (50/50) specificity. We obtained concordant results in 75 samples (98.7%) and discrepant results in one sample (1.32%) between the DG Gel and DiaMed-ID for antibody identification.

CONCLUSION:

Both the microtube column agglutination systems work well and showed high estimated sensitivity and specificity with high concordance. Therefore, the DG gel microtube column agglutination system can be used with good results.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cruz Roja / Prueba de Coombs / Tamizaje Masivo / Sensibilidad y Especificidad / Aglutinación / Corea (Geográfico) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje País/Región como asunto: Asia Idioma: Coreano Revista: Korean Journal of Blood Transfusion Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cruz Roja / Prueba de Coombs / Tamizaje Masivo / Sensibilidad y Especificidad / Aglutinación / Corea (Geográfico) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje País/Región como asunto: Asia Idioma: Coreano Revista: Korean Journal of Blood Transfusion Año: 2007 Tipo del documento: Artículo