Evaluation of BiosewoomTM Real-Q Cytomegalovirus Quantification kit for Cytomegalovirus Viral Load Measure / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 298-304, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-144485
ABSTRACT
BACKGROUND:
Rapid and accurate laboratory tests are essential to detect cytomegalovirus (CMV) infections in solid organs and haematopoietic stem cell transplant recipients. We assessed the realtime quantitative PCR (RQ-PCR) technology for its usefulness in detecting CMV DNA.METHODS:
We evaluated the analytical performance of CMV RQ-PCR using Real-Q Cytomegalovirus Quantification kit (BioSewoom Inc., Korea). To evaluate its clinical utility, we also compared it to pp65 antigenemia test, an immunostaining method, on 343 samples of total 84 patients, including 63 transplant recipients.RESULTS:
The detection limit of RQ-PCR was 63 copies/mL and none of hepatitis B virus, hepatitis C virus, or human immunodeficiency virus showed a cross-reactivity with CMV. Total coefficient of variation (CV) was 10.4-19.5%. It detected CMV DNA in a linear range from 1 x 10(2) to 5 x 10(11) copies/mL (P<10(-13), R2=0.9994). The qualitative positive rates of pp65 antigenemia test and RQ-PCR were 4.7%, 16.3%, respectively and concordance rate between the two tests was 84.8% (K=0.221, P<10(-6)). In comparison of quantitative results, the correlation between two tests was significant (r=0.45, P<10(-17)). In comparison among three groups by pp65 antigen level, CMV DNA level obtained with RQ-PCR increased significantly (P<10(-3) and P<10(-7), respectively).CONCLUSIONS:
The RQ-PCR is easier to perform than the immunostaining method, has good analytical performance and reflects the blood level of viral DNA well. It may be a new method substituting the pp65 antigenemia test. Further studies determining RQ-PCR value starting pre-emptive therapy will be required.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfoproteínas
/
Juego de Reactivos para Diagnóstico
/
ADN Viral
/
Proteínas de la Matriz Viral
/
Reacción en Cadena de la Polimerasa
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Infecciones por Citomegalovirus
/
Carga Viral
/
Citomegalovirus
Tipo de estudio:
Estudio diagnóstico
/
Investigación cualitativa
Límite:
Adolescente
/
Adulto
/
Anciano
/
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
/
Recién Nacido
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Año:
2007
Tipo del documento:
Artículo
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