Effects of dehydroepiandrosterone on Th2 cytokine production in peripheral blood mononuclear cells from asthmatics
The Korean Journal of Internal Medicine
;
: 176-181, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-147571
ABSTRACT
BACKGROUND/AIMS:
The androgen dehydroepiandrosterone (DHEA) attenuates allergic inflammatory airway reactions by down-regulating the Th2 response in mice. The purpose of this study was to investigate whether DHEA suppresses Th2 cytokine production in cultured peripheral blood mononuclear cells (PBMCs) from asthmatic patients.METHODS:
Sixty-one consecutive suspected asthmatic or non-asthmatic men underwent tests for asthma. PBMCs from each subject were cultured with and without DHEA (0.01~10 micrometer) for 48 h. The concentrations of interferon (IFN)-gamma, interleukin (IL)-5, and IL-10 in the culture supernatant were measured via an enzyme-linked immunosorbent assay.RESULTS:
In PBMCs from subjects exhibiting methacholine airway hyperresponsiveness (AHR), DHEA significantly suppressed IL-10, IL-5, and IFN-gamma production in a dose-dependent manner (all p<0.001) and tended to increase the IFN-gamma/IL-5 ratio (p=0.087). DHEA (10 micrometer) suppressed cytokine production to a greater degree in subjects with AHR compared with those without AHR (IL-5 24.0+/-7.8% vs. 40.9+/-3.6%, p<0.01; IFN-gamma 29.7+/-7.0% vs. 54.5+/-5.1%, p<0.01). Cytokine suppression was significantly related to AHR, serum total IgE levels, and skin reactivity to house dust mites.CONCLUSIONS:
DHEA suppressed both Th1 and Th2 responses, with a Th1 bias, and the degree of suppression was associated with the severity of AHR or atopy. Therefore, DHEA may be a useful therapy for asthma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Asma
/
Adyuvantes Inmunológicos
/
Citocinas
/
Deshidroepiandrosterona
/
Células Th2
/
Técnicas de Cultivo de Célula
Límite:
Adolescente
/
Adulto
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
The Korean Journal of Internal Medicine
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS