Effects of Vero Cells Co-culture System on The In Vitro Development of Mouse Preimplantation Embryos in Media with Different Composition of Glucose and Pyruvate / 대한산부인과학회지

ABSTRACT

OBJECTIVE: The purpose of this study was to examine the effects of vero cells co-culture system on the in vitro development of mouse preimplantation embryos in culture media with different composition of glucose and pyruvate. METHODS: Two-cell embryos were collected from 4-5 weeks old ICR mice. Seven hundreds twenty two embryos were cultured with or without vero cells monolayer in four media with different compositions that was manufactured by two DMEM media with (DMEM-GGP) or without (DMEM-G) glucose and pyruvate. In control, DMEM-G medium which is currently using for human embryo culture in our infertility clinic was used. Group I (DMEM-G(1/4)GP) was cultured in medium which was mixed three volume of DMEM-G and one volume of DMEM-GGP, and group II (DMEM-G(1/2)GP) was cultured in medium which was mixed same volume of DMEM-G and DMEM-GGP, and group III was cultured in DMEM-GGP. All media were added to 20% hFF. Results between different groups were analyzed using a Chi-square test, and considered statistically significant when p value was less than 0.05. RESULTS: The developmental rate into 3-cell in vitro culture by each different DMEM media without vero cell, group I (90.2%) was higher than other groups. And also, the rate of embryonic development after 48 (morula blastocyst blastocyst culture, group I (85.9, 89.1 and 69.6%, respectively) was significantly (p<0.05) higher than other groups. In the rate of development on in vitro co-culture by each different DMEM media with vero cell, group III (97.8, 96.7 and 65.6%, respectively) was significantly (p<0.05) higher after 24, 48 and 96hrs in culture than other groups. As for the rate of embryonic development into blastocyst culture, group I (61.1%) and II (64.4%) were significantly higher than group I (45.4%) and IV (20.4%).

CONCLUSION:

In culture system using only each different DMEM media without vero cell, group II was more effective for the embryonic development than other groups. However, in co-culture system by each different DMEM media with vero cell, group III was more effective than other groups. These results are likely that was caused by consumption on glucose and pyruvate of vero cells in culture media. Therefore, the composition of energy sources in culture media may be very important point in co-culture system.