Suppressive effect of dehydroepiandrosterone on the development of asthmatic reaction in BALB/c mice / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
;
: 116-126, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-14979
ABSTRACT
BACKGROUND:
Dehydroepiandrosterone (DHEA), an adrenal androgen, may regulate Th1/Th2 cytokines.OBJECTIVE:
To determine whether DHEA have a preventive effect against the development of asthma, a Th2-associated disease. MATERIALS ANDMETHODS:
Female BALB/c mice were fed with a standard diet incorporated with 0.1% (w/w) of DHEA for 1 week before sensitization with ovalbumin (OVA, 20 microgram i.p. twice with 2-week intervals). Enhanced pause (Penh) was monitored after 1% OVA inhalations for 3 successive days, and then the methacholine bronchoprovocation tests were performed using a whole-body plethysmography system. Analyses of inflammatory cells, cytokines, and OVA-specific IgE were carried out.RESULTS:
Both the excessive airway narrowing (maximum Penh) and the airway hypersensitivity (PC200, mg/mL) to methacholine were significantly suppressed with the DHEA pretreatment (P<.05 and P<.01, respectively). However, the numbers of the inflammatory cells and the levels of interleukin (IL)-4, IL-5, and interferon (IFN)-gamma in the bronchoalveolar lavage fluid, the production of IL-4, IL-5, and IFN-gamma by Con A-stimulated splenocytes, and the serum IgE level in DHEA group were not significantly different from those in asthma group.CONCLUSION:
DHEA pretreatment could suppress the development of asthmatic airway hyperresponsiveness without suppression of Th2 cytokines and airway inflammation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Óvulo
/
Pletismografía
/
Asma
/
Inmunoglobulina E
/
Líquido del Lavado Bronquioalveolar
/
Ovalbúmina
/
Citocinas
/
Cloruro de Metacolina
/
Interleucinas
/
Interferones
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Journal of Asthma, Allergy and Clinical Immunology
Año:
2004
Tipo del documento:
Artículo
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