Chemokine Signaling Pathway Involved in CCL2 Expression in Patients with Rheumatoid Arthritis
Yonsei Medical Journal
;
: 1134-1142, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-150468
ABSTRACT
PURPOSE:
Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progression of which leads to the destruction of cartilage and bone. Chemokines are involved in RA pathogenesis. In this study, we investigated the chemokine signaling pathway associated with CCL2 in peripheral blood (PB) and synovial tissues (ST) of RA patients based on our previous work about chemokine signaling pathway involved in the activation of CCL2 production in collagen-induced arthritis rat ST. MATERIALS ANDMETHODS:
Total RNA was isolated from PB leukocytes and synovium of the knee joint in both RA patients and control populations. Real-time polymerase chain reaction was used to determine CCL4, CCR5, c-Jun, c-Fos, and CCL2 expressions. Serum level of CCL2 was assessed by enzyme-linked immunosorbent assay, and the production of CCL2 in ST was analyzed immunohistochemically.RESULTS:
The expressions of CCL4, CCR5, c-Jun, c-Fos, and CCL2 messenger RNA in RA patients were significantly higher than those in healthy controls, both in ST and on PB leukocyte. Serum CCL2 levels were elevated in RA patients. Histological examination of rheumatoid joints revealed extensive CCL2 expression in RA ST.CONCLUSION:
CCL2, CCL4, c-Jun, c-Fos, and CCR5 may play an important role in the recruitment of PB leukocytes into the RA joints. These data provide evidence that the chemokine signaling pathway is involved in CCL2 expression in RA patient tissues, which may contribute to chronic inflammation associated with RA. Targeting this signaling pathway may provide a novel therapeutic avenue in RA.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Artritis Reumatoide
/
Membrana Sinovial
/
ARN Mensajero
/
Ensayo de Inmunoadsorción Enzimática
/
Transducción de Señal
/
Estudios de Casos y Controles
/
Expresión Génica
/
Quimiocina CCL2
/
Quimiocinas
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Estudio observacional
/
Factores de riesgo
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2015
Tipo del documento:
Artículo
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