Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence
The Korean Journal of Internal Medicine
;
: 883-889, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-151261
ABSTRACT
BACKGROUND/AIMS:
We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence.METHODS:
HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot.RESULTS:
Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA.CONCLUSIONS:
This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Envejecimiento
/
Western Blotting
/
Southern Blotting
/
Telómero
/
Senescencia Celular
/
Células Mesangiales
/
Caveolina 1
/
Glucosa
Límite:
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Internal Medicine
Año:
2017
Tipo del documento:
Artículo
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