Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone) / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 115-123, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-151308
ABSTRACT
OBJECTIVE:
To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II.METHOD:
Human endometrial cancer cells (HEC1A) were cultured under the following condition DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins.RESULTS:
GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin beta3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II.CONCLUSION:
GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Timidina
/
Immunoblotting
/
Transducción de Señal
/
Hormona Liberadora de Gonadotropina
/
Neoplasias Endometriales
/
Integrina beta3
/
Proliferación Celular
Límite:
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Año:
2006
Tipo del documento:
Artículo
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