Expression of Mouse Homolog of Glucosamine-6-Phosphate Deaminase in Reproductive Organs / 대한남성과학회지
Korean Journal of Andrology
;
: 41-46, 2000.
Artículo
en Coreano
| WPRIM
| ID: wpr-154601
ABSTRACT
PURPOSE:
To verify the expression of the mouse homolog of glucosamine-6-phosphate deaminase (GNPDA) in the testis and reproductive organs. MATERIALS ANDMETHODS:
Expression of GNPDA was examined using a polyclonal antibody raised against a synthetic oliogopeptide of the N-terminus of the protein.RESULTS:
In Western blots, an immunoreactive band of Mr 35 kDa was detected in the testis, ovary, and uterus extracts. Expression of GNPDA was greater in the adult than in the immature testis. With immunostaining, a positive signal was found in the cytoplasm of interstitial, Sertoli, and germ cells of adult testis. In the ovary, positive staining was found in the interstitial and luteal cells.Conclusion:
The expression of GNPDA in many reproductive tissues suggests that the enzyme plays a housekeeping role in cell physiology and in the differentiation of the seminiferous tubules.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ovario
/
Túbulos Seminíferos
/
Testículo
/
Útero
/
Fenómenos Fisiológicos Celulares
/
Western Blotting
/
Citoplasma
/
Células Germinativas
/
Tareas del Hogar
/
Células Lúteas
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Andrology
Año:
2000
Tipo del documento:
Artículo
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