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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR
The Korean Journal of Parasitology ; : 573-577, 2013.
Artículo en Inglés | WPRIM | ID: wpr-155353
ABSTRACT
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Porcinos / Enfermedades de los Porcinos / Colorantes Azulados / Toxoplasma / Bioensayo / Encéfalo / Toxoplasmosis Animal / ADN Protozoario / Sensibilidad y Especificidad / Parasitemia Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Porcinos / Enfermedades de los Porcinos / Colorantes Azulados / Toxoplasma / Bioensayo / Encéfalo / Toxoplasmosis Animal / ADN Protozoario / Sensibilidad y Especificidad / Parasitemia Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2013 Tipo del documento: Artículo