Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes
Nutrition Research and Practice
;
: 160-165, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-156149
ABSTRACT
3T3-L1 preadipocyte were differentiated to adipocytes, and then treated with 0, 10, 20, and 40 microg/mL of peanut sprout ethanol extract (PSEE). The main component of PSEE is resveratrol which contained 5.55 mg/mL of resveratrol. The MTT assay, Oil-Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, and the triglyceride concentration were determined in 3T3-L1 cells. MMP-2 and MMP-9 activities as well as mRNA expressions of C/EBP beta and C/EBP alpha were also investigated. As the concentration of PSEE in adipocytes increased, the cell proliferation was decreased in a dose-dependent manner from 4 days of incubation (P < 0.05). The GDPH activity (P < 0.05) and the triglyceride concentration (P < 0.05) were decreased as the PSEE treatment concentration increased. The mRNA expression of C/EBPbeta in 3T3-L1 cells was significantly low in groups of PSEE-treated, compared with control group (P < 0.05). The MMP-9 (P < 0.05) and MMP-2 (P < 0.05) activities were decreased in a dose-dependent manner as the PSEE concentration increased from 20 microg/mL. In conclusion, it was found that PSEE has an effect on restricting proliferation and differentiation of adipocytes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estilbenos
/
ARN Mensajero
/
Adipocitos
/
Metaloproteinasas de la Matriz
/
Células 3T3-L1
/
Proliferación Celular
/
Etanol
/
Fibroblastos
/
Glicerolfosfato Deshidrogenasa
Límite:
Animales
Idioma:
Inglés
Revista:
Nutrition Research and Practice
Año:
2013
Tipo del documento:
Artículo
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