Construction of EST Database for Comparative Gene Studies of Acanthamoeba
The Korean Journal of Parasitology
;
: 103-107, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-156347
ABSTRACT
The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported cDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http//www.amoeba.or.kr).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acanthamoeba
/
Etiquetas de Secuencia Expresada
/
Bases de Datos Genéticas
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS