HPV in situ hybridization and immunohistochemical expression of L1 capsid protein in cervical intraepithelial neoplasia / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 1279-1286, 2009.
Artículo
en Coreano
| WPRIM
| ID: wpr-156459
ABSTRACT
OBJECTIVE:
HPV in situ hybridization (ISH) is able to detect HPV DNA and identify integrated HPV DNA by punctuate staining and episomal HPV by diffuse staining in the nuclei. Because the expression of L1 capsid protein disappears after integration of HPV DNA, immunohistochemistry (IHC) of L1 capsid protein can be used as a indirect evidence of integration. Therefore, we tried to evaluate the usefulness of HPV ISH and IHC of L1 capsid protein in Cervical intraepithelial neoplasia (CIN).METHODS:
Twenty six cervical lesions from patients with CIN and 19 normal cervical epithelium from patients with leiomyoma were evaluated with HPV ISH and IHC of L1 capsid protein.RESULTS:
HPV ISH was positive in 80.8% (21/26) in CIN. Among 21 positive cases, diffuse staining was observed in 42.9% and punctuated and diffuse staining in 57.1%. L1 capsid protein was positive in 65.4% (17/26) of cervical tissue sections and 15.4% (4/26) of cervical smears. The punctuated staining of HPV ISH was correlated with high grade CIN (P=0.007) but expression of L1 capsid protein was not associated with grade of CIN (P>0.05).CONCLUSION:
HPV ISH is a useful tool to identify integrated HPV DNA in paraffin-embedded, formalin-fixed cervical tissue. HPV integration confirmed by HPV ISH was associated with high-grade CIN. IHC of L1 capsid protein showed better result using cytology smears than tissue sections.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Frotis Vaginal
/
ADN
/
Inmunohistoquímica
/
Displasia del Cuello del Útero
/
Cápside
/
Hibridación in Situ
/
Proteínas de la Cápside
/
Epitelio
/
Leiomioma
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2009
Tipo del documento:
Artículo
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