The effect of Insulin like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) on cellular proliferation in mouse 3T3 fibroblast cells / 결핵
Tuberculosis and Respiratory Diseases
;
: 618-628, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-157765
ABSTRACT
BACKGROUND:
Cell growth is a balance between cell proliferation and cell death. Insulin-like growth factor-I(IGF-I), which binds IGF-I receptor (IGF-IR), mediates cellular proliferation as a potent mitogen. IGF binding protein-3 (IGFBP-3) as a circulating major IGFBP can inhibit or enhance the effects of IGF-I on cellular growth by binding IGFs.METHODS:
We investigated the expressions of mRNA of IGF-I and IGF-IR by northern blot and phosphorylation of IGF-IR with the treatment of IGF-I by western blot in 3T3 fibroblast cells. The cellular proliferations of 3T3 cells with the treatments of IGF-I were evaluated using 3H-thymidine incorporation and MTT assay. Also to observe the effect of IGFBP-3 on cellular proliferation, 3T3 cells were treated with anti-IGFBP-3 and alpha IR3(monoclonal antibody to IGF-IR) alone or in combination.RESULTS:
Our results demonstrated that 3T3 cells showed mRNA expressions of IGF-I and IGF-IR and the IGF-I increased phosphorylation of IGF-IR. The treatments of 3T3 cells with IGF-I increased cellular proliferation in 5% and 1% seruma-containing media, not in serum-free media. The addition of anti-IGFBP-3 to neutralize IGFBP-3 showed 2-fold increase of cellular proliferation, and also co-incubation of anti-IGFBP-3 and alpha IR3 together showed similar increase of cellular proliferation in 3T3 cells. Interestingly, when the cells were prethreated with alpha IR3 for 4 hr, prior to the simultaneous addition of alpha IR3 and anti-IGFBP-3, anti-IGFBP-3-mediated cellular proliferation was decreased to control level. All of these results suggest that free IGF-I released from IGF-I/IGFBP-3 complex would be involved in the cellular proliferation.CONCLUSION:
IGF-I action by binding IGF-I.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
ARN Mensajero
/
Factor I del Crecimiento Similar a la Insulina
/
Western Blotting
/
Northern Blotting
/
Células 3T3
/
Medio de Cultivo Libre de Suero
/
Muerte Celular
/
Receptor IGF Tipo 1
/
Proteínas de Unión a Factor de Crecimiento Similar a la Insulina
Límite:
Animales
Idioma:
Coreano
Revista:
Tuberculosis and Respiratory Diseases
Año:
1999
Tipo del documento:
Artículo
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