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Comparison of Efficacy of Human Papilloma Virus Genotyping Assays using Restriction Fragment Mass Polymorphism and DNA Chip Analysis in Patients with Abnormal Pap Smear and Uterine Cervical Cancer
Korean Journal of Pathology ; : 439-447, 2006.
Artículo en Coreano | WPRIM | ID: wpr-157922
ABSTRACT

BACKGROUND:

High-risk human papilloma virus (HPV) infection is the primary cause of cervical cancer; there is a need for more sensitive and reliable methods for HPV genotyping to use as screening tools for early detection and intervention.

METHODS:

A novel MALDI-TOF MSbased assay, termed Restriction Fragment Mass Polymorphism (RFMP) was developed for multiple HPV genotyping. Its performance was compared with DNA chip technology. The study was based on 164 cases classified as normal (n=40), ASCUS (n=53) and invasive squamous cell carcinoma (SCC, n=71) by a PAP smear and/or cervical colposcopic biopsy.

RESULTS:

High-risk genotypes were detected in 7.5%, 47.2% and 97.2% in normal, ASCUS and SCC groups by RFMP, and in 20.0%, 41.5% and 90.1% using DNA chip technology, respectively. The results showed substantial concordance, with a kappa coefficient of 0.688, between the methods. Diagnostic sensitivity and specificity for cervical cancer were found to be 97.2% and 92.2% with RFMP and 90.1% and 80.0% using DNA chip microarrays.

CONCLUSIONS:

RFMP and DNA chip technologies were shown to be reliable methods for HPV genotyping with a high concordance. The improved sensitivity and specificity should make RFMP a viable option for the management of women with cervical neoplastic lesions.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papiloma / Biopsia / ADN / Carcinoma de Células Escamosas / Neoplasias del Cuello Uterino / Tamizaje Masivo / Sensibilidad y Especificidad / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Análisis de Secuencia por Matrices de Oligonucleótidos / Genotipo Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Límite: Femenino / Humanos Idioma: Coreano Revista: Korean Journal of Pathology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Papiloma / Biopsia / ADN / Carcinoma de Células Escamosas / Neoplasias del Cuello Uterino / Tamizaje Masivo / Sensibilidad y Especificidad / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Análisis de Secuencia por Matrices de Oligonucleótidos / Genotipo Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Límite: Femenino / Humanos Idioma: Coreano Revista: Korean Journal of Pathology Año: 2006 Tipo del documento: Artículo