Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells
Experimental & Molecular Medicine
;
: 240-245, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-159763
ABSTRACT
We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas de Precipitina
/
Acetato de Tetradecanoilforbol
/
Células Tumorales Cultivadas
/
Transfección
/
Regulación de la Expresión Génica
/
Northern Blotting
/
Fibronectinas
/
Regiones Promotoras Genéticas
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
1998
Tipo del documento:
Artículo
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