Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
Biomolecules & Therapeutics
;
: 315-320, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-160698
ABSTRACT
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Autofagia
/
Naranja de Acridina
/
Especies Reactivas de Oxígeno
/
Colon
/
Neoplasias del Colon
/
Microscopía Confocal
/
Cisteína
/
Citometría de Flujo
/
Fluorouracilo
/
Microscopía
Idioma:
Inglés
Revista:
Biomolecules & Therapeutics
Año:
2017
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS