Synthetic CDCA Derivatives-Induced Apoptosis of Stomach Cancer Cell Line SNU-1 Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 132-139, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-162441
ABSTRACT
PURPOSE:
This study was conducted to explore whether CDCA derivatives induce apoptosis in a stomach cancer cell line, and to dissect the detailed mechanism underlying apoptosis. MATERIALS ANDMETHODS:
The human stomach cancer cell line, SNU-1, cells were treated with the synthetic CDCA derivatives, HS-1199 and HS-1200. DNA and mitochondrial stains were used to detect apoptotic cells by fluorescence imaging or flow cytometry. The caspase-3 activity was measured by Western blotting.RESULTS:
Both the HS-1199 and HS-1200 induced decreased viabilities of the SNU-1 cells, in time-dependent manners. The CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes reduction of MMP, cytochrome c release, and Smac/ DIABLO translocation), activation of caspase-3 (resulting in the degradation of PARP and DFF45), DNA fragmentation and nuclear condensation.CONCLUSION:
The CDCA derivatives, HS-1199 and HS- 1200, both induced apoptosis of the SNU-1 gastric cancer cells in caspase- and mitochondria-dependent fashions. Many important issues relating to their therapeutic applications remain to be elucidated.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estómago
/
Neoplasias Gástricas
/
ADN
/
Línea Celular
/
Ácido Quenodesoxicólico
/
Western Blotting
/
Apoptosis
/
Caspasas
/
Citocromos c
/
Colorantes
Límite:
Humanos
Idioma:
Inglés
Revista:
Cancer Research and Treatment
Año:
2004
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS