Lithium Potentiates the FeCl2 Induced Free Radical Injury in Primary Mouse Cortical Cell Culture / 대한정신약물학회지
Korean Journal of Psychopharmacology
;
: 468-474, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-163631
ABSTRACT
OBJECTIVES:
For the past half century, lithium has been used for the acute and prophylactic treatment of bipolar disorder and recurrent depression. Recently, new pharmacological effects of Li+ have appeared, showing that Li+ can influence neuronal injury. We tested the effects of Li+ on free radical induced neuronal injury in primary murine cortical cell cultures.METHODS:
Cortical cells were prepared from fetal mice (embryonic day 15) and exposed to 30 micrometer Fe2+ alone or with 5 mM Li+ or 5 mM Li+ alone for 24 hrs at Days in vitro (DIV) 14. Neuronal death was analyzed by measuring lactate dehydrogenase (LDH) release into media. The fluorescence of 2',7'-dichlorofluorescin (DCF) was measured in as a mean of estimating the formation of reactive oxygen species (ROS).RESULTS:
Li+ alone does not produce neuronal injury itself but it potentiates Fe2+-induced neuronal injury through increasing the production of free radical.CONCLUSION:
This study suggests that the effects of Li+ on neuronal survivorship may be injury type dependent and Li+ potentiate the free radical injury. Therefore in practice clinician should be cautious in using the lithium in the treatment of brain injured patients.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Trastorno Bipolar
/
Encéfalo
/
Tasa de Supervivencia
/
Especies Reactivas de Oxígeno
/
Técnicas de Cultivo de Célula
/
Depresión
/
Fluorescencia
/
L-Lactato Deshidrogenasa
/
Litio
/
Necrosis
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Psychopharmacology
Año:
2006
Tipo del documento:
Artículo
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