Regulation of type I collagen and interstitial collagenase mRNA expression in human dermal fibroblasts by colchicine and D-penicillamine
Yonsei Medical Journal
;
: 490-495, 1999.
Artículo
en Inglés
| WPRIM
| ID: wpr-164914
ABSTRACT
Sclerosis is a disease process in which idiopathic hardening occurs in the skin and/or internal organs as a result of the accumulation of type I collagen, induced mainly by transforming growth factor-beta. Colchicine and D-penicillamine are widely used for its treatment. Their effects are known to be due to post-translational down-regulation of type I collagen synthesis, with colchicine also up-regulating interstitial collagenase. To determine whether or not they have any pre-translational effect on type I collagen and MMP-1, and also to observe their effects on the action of TGF-beta, cultured neonatal foreskin fibroblasts were treated with colchicine and D-penicillamine, singly and together. The amount of type I collagen and MMP-1 mRNA were quantitated by Northern blot hybridization. Colchicine suppresses the basal level of type I collagen mRNA but minimally stimulates the mRNA expression of MMP-1, whereas D-penicillamine does not have any significant effects on either. Colchicine was also able to significantly suppress the TGF-beta-induced up-regulation of type I collagen mRNA expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Penicilamina
/
Piel
/
ARN Mensajero
/
Células Cultivadas
/
Colchicina
/
Regulación de la Expresión Génica
/
Factor de Crecimiento Transformador beta
/
Colágeno
/
Metaloproteinasa 1 de la Matriz
/
Fibroblastos
Límite:
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
1999
Tipo del documento:
Artículo
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