Flow Cytometric AHG-CDC for HLA Crossmatch: A Pilot Study / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 122-131, 2010.
Artículo
en Coreano
| WPRIM
| ID: wpr-165962
ABSTRACT
BACKGROUND:
For HLA crossmatch in organ transplantation, complement-dependent cytotoxicity (CDC) is a very useful methodology to detect donor-specific HLA antibodies and their complement fixing ability. In this preliminary pilot study, we investigated whether dead cells induced in anti-human globulin (AHG)-augmented CDC (AHG-CDC) reaction could be measured by flow cytometry ('FC AHG-CDC').METHODS:
This FC AHG-CDC measured the percentage of dead cells (% dead cells) after 7-aminoactinomycin D staining using 3 color flow cytometry. A total 45 flow cytometry crossmatch (FCXM) cases of 12 positives and 33 negatives were further tested using this FC AHG-CDC.RESULTS:
The % dead cells of FC AHG-CDC was significantly correlated with the mean fluorescent intensity ratio of FCXM (T cells r=0.613, P=7.45x10(-6); B cells r=0.404, P=0.006). The positivity rate of FC AHG-CDC among FCXM positive cases was relatively high 80% (8/10) for T cells and 75% (9/12) for B cells. The negativity rate of FC AHG-CDC among FCXM negative cases was 100% (35/35) for T cells and 91% (30/33) for B cells.CONCLUSION:
In this pilot study, FC AHG-CDC could yield quantitative values, % dead cells, which was proportional to the level of complement-fixing cytotoxic antibodies against T and B cells, respectively, even without physical separation of cells or serial dilution of serum.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas del Sistema Complemento
/
Linfocitos B
/
Linfocitos T
/
Proyectos Piloto
/
Trasplante de Órganos
/
Trasplantes
/
Dactinomicina
/
Citometría de Flujo
/
Anticuerpos
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS