The Regulation of OPG/OCIF mRNA Epression by IL-1beta in Peripheral Blood Mononuclear Cells / 대한내분비학회지
Journal of Korean Society of Endocrinology
;
: 204-213, 2000.
Artículo
en Coreano
| WPRIM
| ID: wpr-166421
ABSTRACT
BACKGROUND:
Osteoprotegerin(OPG) is a soluble member of the tumor necrosis factor(TNF) receptor family and inhibits osteoclastogenesis by interrupting the cell-to-cell interaction between osteoblastic/stromal cells and osteoclast progenitors. OPG is expressed in many tissues including osteoblasts and may act on bone tissues in a paracrine and/or autocrine fashion. Futhermore, many cytokines and growth factors are known to influence the regulation of OPG expression in osteoblastic/stromal cells. The aims of the present study were to examine whether or not OPG was expressed in human peripheral blood mononuclear cells(PBMCs) and to investigate the effects of IL-1beta, which were known as potent osteotropic agents, on the regulation of OPG mRNA in PBMCs.METHODS:
PBMCs were isolated by centrifugation over Ficoll-Hypaque density gradients from postmenopausal women and cultured in 6-well plates containing alpha-MEM supplemented with 5% FBS. The expression of OPG mRNA in PBMCs was observed by RT-PCR in adherent and nonadherent cells on culture plates. To observe the effect of OPG expression by IL-1beta, we measured the concentration of OPG mRNA by altering the concentration and incubation time of IL-1beta. The measurement of OPG mRNA was done by semi-quantitative PCR and indicated as OPG/GAPDH.RESULTS:
OPG was expressed both in cells attached to the surface of culture plates and in non-adherent cells for the incubation of peripheral blood mononuclear cells. The effect of OPG mRNA by IL-1beta tend to increase in accordance with the length of incubation time and maximizes at 12 hours of incubation time and shows 1.2-3.5 times higher than the standard level at the concentration of 0.5ng/ml. However, the increased quantity in concentration varies according to individuals.]CONCLUSION:
OPG mRNA is expressed in peripheral blood mononuclear cells and known to be increased by IL-1beta.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Osteoclastos
/
Huesos
/
ARN Mensajero
/
Comunicación Celular
/
Centrifugación
/
Reacción en Cadena de la Polimerasa
/
Citocinas
/
Péptidos y Proteínas de Señalización Intercelular
/
Osteoprotegerina
Límite:
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Journal of Korean Society of Endocrinology
Año:
2000
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS