In Vitro IL-1, TNF and IL-6 Production of Peripheral Blood Mononuclear Cells Stimulated with Different Hemodiaysis Membranes / 대한내과학회지
Korean Journal of Medicine
;
: 814-822, 1997.
Artículo
en Coreano
| WPRIM
| ID: wpr-166461
ABSTRACT
OBJECTIVES:
In order to evaluate the role of these cytokines in biological response induced by blood interaction with hemodialysis membranes.METHODS:
We have investigated the IL-1, TNF and IL-6 concentrations in the supernatant of 24-hours cultured peripheral blood mononuclear cells (PBMC) without(spontaneous group) or with broken cuprophan or P1VMA membranes in 9 chronic hemodialyzed patients and 8 healthy controls. The blood samples were drawn before dialysis using following criteria (a) in last dialytic treatment with PMMA membranes(HDEl), (b) after two weeks of dialytic treatment wih cuprophan membranes(HDE2).RESULTS:
In the both of patient group(HDE1 and HDE2) and controls production of IL-l, TNF and IL-6 of PBMC stimulated with cuprophan or PMMA membrane particles was increased compared to those of spontaneous group. IL-1 production of HDE1 stimulated PMMA(99.31 +/- 30.15fmol/ml) was significantly higher compared to that of cuprophan(48.43 +/- 11.29fmol/ml), TNF production of HDE2 with cuprophan(114.86 +/- 38.5lfmoVml) was significantly high compared to that of spontaneous group(52.42 +/- 29.94fmol/ml). IL-6 production of HDE2(646.70 +/- 103.84fmol/ml) was significantly high compared to that of spontaneous group(385.88 +/- 87.03fmoVml). Comparing cytokine production of PBMC, there was a significant correlation between IL-1 and IL-6(r=0.78), IL-1 and TNF(r=0.78) and TNF and IL-6(r=0,76).CONCLUSION:
Our results show that the interaction of cuprophan or PMMA membranes with blood increase the production of IL-1, TNF and IL-6. We suggest that in patients undergoing routine hemodialysis PBMC are primed by exposure to chronic stimulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Citocinas
/
Diálisis Renal
/
Interleucina-6
/
Interleucina-1
/
Polimetil Metacrilato
/
Diálisis
/
Membranas
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Medicine
Año:
1997
Tipo del documento:
Artículo
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