Proliferation Patterns of N-butyl-N- ( 4-hydroxybutyl ) Nitrosamine ( BBN ) Induced Rat Bladder Tumor Identified by Histone mRNA In Situ Hybridization / 대한비뇨기과학회지
Korean Journal of Urology
;
: 111-119, 1997.
Artículo
en Coreano
| WPRIM
| ID: wpr-166830
ABSTRACT
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifty fold then rapidly disappears at the start of G2-phase. The presence, therefore, of abundant quantities of histone mRNA provides a molecular marker of cycling cells. The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black cytoplasmic staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta,T1). The higher S-phase fraction indicated greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Vejiga Urinaria
/
Neoplasias de la Vejiga Urinaria
/
ARN Mensajero
/
Histonas
/
Ciclo Celular
/
Hibridación in Situ
/
Citoplasma
/
Replicación del ADN
/
Carcinogénesis
/
Hiperplasia
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Urology
Año:
1997
Tipo del documento:
Artículo
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