Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China
Journal of Veterinary Science
;
: 523-529, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-167766
ABSTRACT
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rumiantes
/
Cabras
/
Ovinos
/
ARN Ribosómico 16S
/
China
/
Reacción en Cadena de la Polimerasa
/
Epidemiología
/
Prevalencia
/
Análisis de Secuencia de ADN
/
Anaplasma phagocytophilum
Tipo de estudio:
Estudio de prevalencia
Límite:
Animales
/
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2016
Tipo del documento:
Artículo
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