Establishment of Mouse Oocytes Cryopreservation Program / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1455-1463, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-167799
ABSTRACT
OBJECTIVE:
To establish the optimal cryopreservation method in mouse oocytes.METHODS:
Firstly, mouse immature oocytes were exposed to various cryoprotectants, and then cryoprotectant with the best outcome was selected. Secondly, mouse immature oocytes were cryopreserved by either slow freezing and ultra-rapid thawing or vitrification. Finally, in mouse mature oocytes, the five different protocols were compared in their fertilization and hatching rates.RESULTS:
1) 1.5M 1,2-propanediol (PROH) and 1.5M PROH+0.1M sucrose had a higher rate of survival (73.1%, 81.9%) and in vitro maturation (28.2%, 30.1%). 2) Vitrification using 5.5M ethylene glycol (EG) showed significantly higher rate of survival and in vitro maturation, when compared with slow freezing and ultra-rapid thawing using 1.5M PROH+0.1M sucrose (65.9% vs 50.0%, 40.0% vs 28.2%, respectively). 3) In mouse mature oocytes, vitrification using 5.5M EG showed significantly higher survival rate, however, slow freezing and ultra-rapid thawing using 1.5M DMSO was superior to vitrification in view of fertilization rate.CONCLUSIONS:
Vitrification showed better outcomes in mouse immature oocytes, but slow freezing and ultra-rapid thawing using 1.5M DMSO may be beneficial in mature oocytes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oocitos
/
Sacarosa
/
Criopreservación
/
Dimetilsulfóxido
/
Tasa de Supervivencia
/
Glicol de Etileno
/
Propilenglicol
/
Fertilización
/
Vitrificación
/
Congelación
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2001
Tipo del documento:
Artículo
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