Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry
Journal of Veterinary Science
;
: 107-114, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-169638
ABSTRACT
Despite the development of new technologies, new challenges still remain for large scale proteomic profiling when dealing with complex biological mixtures. Fractionation prior to liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis is usually the preferred method to reduce the complexity of any biological sample. In this study, a gel LC-MS/MS approach was used to explore the stage specific proteome of Cryptosporidium (C.) parvum. To accomplish this, the sporozoite protein of C. parvum was first fractionated using SDS-PAGE with subsequent LC-MS/MS analysis. A total of 135 protein hits were recorded from 20 gel slices (from same gel lane), with many hits occurring in more than one band. Excluding all non-Cryptosporidium entries and proteins with multiple hits, 33 separate C. parvum entries were identified during the study. The overall goal of this study was to reduce sample complexity by protein fractionation and increase the possibility of detecting proteins present in lower abundance in a complex protein mixture.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Protozoarias
/
Cromatografía Liquida
/
Cryptosporidium parvum
/
Proteoma
/
Perfilación de la Expresión Génica
/
Esporozoítos
/
Proteómica
/
Electroforesis en Gel de Poliacrilamida
/
Espectrometría de Masas en Tándem
/
Fraccionamiento Químico
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2013
Tipo del documento:
Artículo
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