Role of HGF/c-Met in Serum-Starved ARPE-19 Cells
Korean Journal of Ophthalmology
;
: 244-250, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-171841
ABSTRACT
PURPOSE:
Hepatocyte growth factor (HGF) and its receptor (HGFR/c-Met) regulate motility, mitogenesis, and morphogenesis in a cell type-dependent fashion. We report the role of HGF and c-Met on stress-induced ARPE-19 human retinal pigment epithelial (RPE) cells in this study.METHODS:
The cells were cultured either with or without serum. Southern and Western blot analyses were done to determine the expression patterns of HGF/c-Met in serum-starved ARPE-19 cells. The cell proliferation pattern in serum-starved condition was analyzed using MTS assay. Inhibition level of cell proliferation was analyzed using a neutralizing monoclonal antibody against c-Met (2 microgram/ml).RESULTS:
Abnormal cell proliferation and scattering of ARPE-19 cells was observed under serum starvation. HGF/c-Met were expressed in serum-starved ARPE-19 cells. ARPE-19 cell proliferation was also enhanced with recombinant HGF treatment. Neutralization against c-Met inhibited the proliferation of serum-deprived ARPE-19 by 64.5% (n=9, S.D. 5.5%). Serum starvation appears to induce epithelial-mesenchymal transition of ARPE-19 cells, resulting in scatter, and the expression of alpha-smooth muscle actin (alpha-SMA), a marker for fibrosis.CONCLUSIONS:
In conclusion, c-Met induced under non-physiologic conditions has significant effects on the activation of RPE cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Epitelio Pigmentado Ocular
/
ARN
/
Expresión Génica
/
Movimiento Celular
/
Células Cultivadas
/
Western Blotting
/
Southern Blotting
/
Reacción en Cadena de la Polimerasa
/
Medio de Cultivo Libre de Suero
/
Factor de Crecimiento de Hepatocito
Límite:
Humanos
Idioma:
Inglés
Revista:
Korean Journal of Ophthalmology
Año:
2007
Tipo del documento:
Artículo
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