Protective Effect of Catechin on Apoptosis of the Lens Epithelium in Rats with N-methyl-N-nitrosourea-induced Cataracts
Korean Journal of Ophthalmology
;
: 101-107, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-171962
ABSTRACT
PURPOSE:
To investigate the effect of catechin on apoptotic cell death in the lens epithelium of rats with cataract.METHODS:
Cataract was induced by intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five groups (n=15 in each group) a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and immunohistochemistry for caspase-3.RESULTS:
Apoptotic cell death in lens epithelial cells that increased following cataract formation in rats was suppressed by cathechin.CONCLUSIONS:
Catechin inhibited cataract-induced apoptotic cell death in the lens epithelium and may prove useful for the prevention of cataract progression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Catarata
/
Distribución Aleatoria
/
Catequina
/
Western Blotting
/
Análisis de Varianza
/
Técnicas para Inmunoenzimas
/
Ratas Sprague-Dawley
/
Apoptosis
/
Etiquetado Corte-Fin in Situ
/
Caspasa 3
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Journal of Ophthalmology
Año:
2010
Tipo del documento:
Artículo
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