Metabolomic analysis of healthy human urine following administration of glimepiride using a liquid chromatography-tandem mass spectrometry
Translational and Clinical Pharmacology
;
: 67-73, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-172329
ABSTRACT
Glimepiride, a third generation sulfonylurea, is an antihyperglycemic agent widely used to treat type 2 diabetes mellitus. In this study, an untargeted urinary metabolomic analysis was performed to identify endogenous metabolites affected by glimepiride administration. Urine samples of twelve healthy male volunteers were collected before and after administration of 2 mg glimepiride. These samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then subjected to multivariate data analysis including principal component analysis and orthogonal partial least squares discriminant analysis. Through this metabolomic profiling, we identified several endogenous metabolites such as adenosine 3′, 5′-cyclic monophosphate (cAMP), quercetin, tyramine, and urocanic acid, which exhibit significant metabolomic changes between pre- and posturine samples. Among these, cAMP, which is known to be related to insulin secretion, was the most significantly altered metabolite following glimepiride administration. In addition, the pathway analysis showed that purine, tyrosine, and histidine metabolism was affected by pharmacological responses to glimepiride. Together, the results suggest that the pharmacometabolomic approach, based on LC-MS/MS, is useful in understanding the alterations in biochemical pathways associated with glimepiride action.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Quercetina
/
Espectrometría de Masas
/
Tirosina
/
Ácido Urocánico
/
Voluntarios
/
Tiramina
/
Análisis de los Mínimos Cuadrados
/
Adenosina
/
Estadística como Asunto
/
Análisis de Componente Principal
Límite:
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Translational and Clinical Pharmacology
Año:
2017
Tipo del documento:
Artículo
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