Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry
Journal of Veterinary Science
;
: 103-108, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-172829
ABSTRACT
Macrolides are frequently used in veterinary medicine as therapeutic and preventive agents for various diseases. It is difficult to determine macrolides simultaneously with conventional methods due to their similar structures. A simultaneous analysis for erythromycin, roxithromycin, tiamulin and tylosin with LC/MS has been developed. Separation was performed on C18 reversed phase column. Mobile phase was gradiently flowed with 10 mM ammonium acetate and methanol. The mass spectrometer was run in the positive mode and selective ion monitoring mode. The molecular ions were [M+H]+ form at m/z 837.5 for erythromycin, at m/z 859.5 for roxithromycin, at m/z 494.2 for tiamulin and at m/z 916.7 for tylosin. Limits of detection were in the range from 0.001 to 0.01 microgram/g lower than their MRLs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrometría de Masas
/
Medicina Veterinaria
/
Estructura Molecular
/
Tilosina
/
Eritromicina
/
Roxitromicina
/
Cromatografía Liquida
/
Sensibilidad y Especificidad
/
Diterpenos
/
Antibacterianos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2002
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS