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The Genetically Modified Polysialylated Form of Neural Cell Adhesion Molecule-Positive Cells for Potential Treatment of X-Linked Adrenoleukodystrophy
Yonsei Medical Journal ; : 246-252, 2013.
Article en En | WPRIM | ID: wpr-17422
Biblioteca responsable: WPRO
ABSTRACT
PURPOSE: Cell transplantation of myelin-producing exogenous cells is being extensively explored as a means of remyelinating axons in X-linked adrenoleukodystrophy. We determined whether 3,3',5-Triiodo-L-thyronine (T3) overexpresses the ABCD2 gene in the polysialylated (PSA) form of neural cell adhesion molecule (NCAM)-positive cells and promotes cell proliferation and favors oligodendrocyte lineage differentiation. MATERIALS AND METHODS: PSA-NCAM+ cells from newborn Sprague-Dawley rats were grown for five days on uncoated dishes in defined medium with or without supplementation of basic fibroblast growth factor (bFGF) and/or T3. Then, PSA-NCAM+ spheres were prepared in single cells and transferred to polyornithine/fibronectin-coated glass coverslips for five days to determine the fate of the cells according to the supplementation of these molecules. T3 responsiveness of ABCD2 was analyzed using real-time quantitative polymerase chain reaction, the growth and fate of cells were determined using 5-bromo-2-deoxyuridine incorporation and immunocytochemistry, respectively. RESULTS: Results demonstrated that T3 induces overexpression of the ABCD2 gene in PSA-NCAM+ cells, and can enhance PSA-NCAM+ cell growth in the presence of bFGF, favoring an oligodendrocyte fate. CONCLUSION: These results may provide new insights into investigation of PSA-NCAM+ cells for therapeutic application to X-linked adrenoleukodystrophy.
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Texto completo: 1 Índice: WPRIM Asunto principal: Ácidos Siálicos / Células Madre / Hormonas Tiroideas / Triyodotironina / Bromodesoxiuridina / Inmunohistoquímica / Diferenciación Celular / Factor 2 de Crecimiento de Fibroblastos / Fibronectinas / Ratas Sprague-Dawley Límite: Animals Idioma: En Revista: Yonsei Medical Journal Año: 2013 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Ácidos Siálicos / Células Madre / Hormonas Tiroideas / Triyodotironina / Bromodesoxiuridina / Inmunohistoquímica / Diferenciación Celular / Factor 2 de Crecimiento de Fibroblastos / Fibronectinas / Ratas Sprague-Dawley Límite: Animals Idioma: En Revista: Yonsei Medical Journal Año: 2013 Tipo del documento: Article