Neuroprotection Effect of Ascorbic Acid in Model of Ischemic Retinal Injury
Journal of the Korean Ophthalmological Society
;
: 1348-1355, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-174563
ABSTRACT
PURPOSE:
An attempt to establish the neuroprotection effect of ascorbic acid in ischemic retinal injury.METHODS:
A rat anterior chamber was cannulated with a 30-gauge infusion needle connected to a container of balanced salt solution (BSS). The container was raised to a height of 3m during 60mins, thereby inducing retina ischemia by high intraocular pressure (HIOP). The effect of ascorbic acid was demonstrated by hematoxylin-eosin (H and E) stain and neuronal nitric oxide synthase (nNOS) immunohistochemical stain. The effect was classified into three HIOP-induced groups without ascorbic acid treatment, ; ascorbic acid-treated at 24 hr and just before experiment; and ascorbic acid-treated at 24hr, just before experiment and every 24hr after experiment.RESULTS:
After retinal ischemia, in the ascorbic acid-treatment groups (Ed- confirm the plural here; from above there are two ascorbic acid-treated groups), the thickness of each retinal layer was preserved more than that in the non-ascorbic acid treated group. There was little difference according to ascorbic acid administration time and period. By nNOS immunohistochemical stain, in the non-ascorbic acid administration group, nNOS immune reactive cells were increased remarkably, in the Inner Nuclear layer (INL) and Ganglion Cell Layer (GCL). In the ascorbic acid-treated groups (Ed-agin confirm), nNOS immune reactive cells were stained in a similar pattern to that of the control group.CONCLUSIONS:
The neuroprotection effect of ascorbic acid in ischemic retinal injury was demonstrated, as was the suppression of nNOS expression in the ischemic retina tissue by ascorbic acid administration.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ácido Ascórbico
/
Retina
/
Retinaldehído
/
Ganglión
/
Óxido Nítrico Sintasa de Tipo I
/
Presión Intraocular
/
Isquemia
/
Cámara Anterior
/
Agujas
Límite:
Animales
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2004
Tipo del documento:
Artículo
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