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Induction of IL-12 Experession in Bone Marrow-derived Mouse Dendritic Cells / 대한면역학회지
Korean Journal of Immunology ; : 121-127, 1999.
Artículo en Coreano | WPRIM | ID: wpr-176071
ABSTRACT
Dendritic cells (DCs) are the most potent antigen presenting cells that can activate naive T cells. Mature DCs exress high levels of MHC and costimulatory molecules on their surface and have capacity to produce IL-12, a 75 kDa heterodimeric cytokine composed of p35 and p40 subunit. IL-12 is currently thought to be one of most critical determinants for skewing the immune response towards Th1. Expression of IL-12 in dendritic cells seems to be regulated by various stimuli including CD40L. In the present study we investigated expression of IL-12 in mature DCs, which were cultured from bone marrow cells in the presence of GM-CSF. Maturity of the DCs was confirmed by morphologic characteristics, immunophenotypes, and allostimulatory activities. Exprssion levels of IL-12 p40 in the DCs were measured by semi-quantitative RT-PCR. Increases in IL-12 p40 expression were observed after treatment with lipopolysaccharide (LPS), an anti-MHC class II monoclonal antibody, or an anti-CD40 monoclonal antibody. The most remarkable increases, however, were observed in the DCs treated with an anti-CD40 monoclonal antibody. These results support a previous notion that signals through CD40/CD40L interaction may be important for the production of IL-12 by DCs. Moreover, results of this study show a possibility of using monoclonal antibodies against CD40 molecules for preparing DCs producing high amount of IL-12, which can be used for anti-tumor or anti-viral immunotherapy.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Dendríticas / Células de la Médula Ósea / Linfocitos T / Factor Estimulante de Colonias de Granulocitos y Macrófagos / Interleucina-12 / Ligando de CD40 / Inmunoterapia / Anticuerpos Monoclonales / Células Presentadoras de Antígenos Límite: Animales Idioma: Coreano Revista: Korean Journal of Immunology Año: 1999 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Dendríticas / Células de la Médula Ósea / Linfocitos T / Factor Estimulante de Colonias de Granulocitos y Macrófagos / Interleucina-12 / Ligando de CD40 / Inmunoterapia / Anticuerpos Monoclonales / Células Presentadoras de Antígenos Límite: Animales Idioma: Coreano Revista: Korean Journal of Immunology Año: 1999 Tipo del documento: Artículo