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Identification of Brucella abortus using the sequencing of omp gene / 대한내과학회지
Korean Journal of Medicine ; : 10-16, 2006.
Artículo en Coreano | WPRIM | ID: wpr-176114
ABSTRACT

BACKGROUND:

As the incidence of bovine brucellosis increases in Korea, the incidence of human brucellosis is also increasing since 2002. However, it is difficult to identify Brucella species by using the conventional methods.

METHODS:

Three strains of gram-negative coccobacilli were isolated from blood specimens of three patients with prolonged fever, which were not identified by using the conventional methods. After extracting total DNA from these isolates, PCR amplification of 16S rRNA and omp2 genes was performed. These sequences secured by PCR assay were compared with known sequences by using GenBank BLAST.

RESULTS:

DNA sequences were obtained from 3 isolates by using PCR amplification of 16S rRNA. These sequences had more than 99.9% similarities with Brucella species by using GenBank BLAST. In the second place, after comparing DNA sequences secured by PCR amplification of omp2a and omp2b by using GenBank BLAST, these isolates were confirmed as B. abortus.

CONCLUSIONS:

DNA sequence analysis is a rapid and accurate method for identification of uncommon microorganisms, such as Brucella species.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Brucella / Brucella abortus / Brucelosis / Brucelosis Bovina / ADN / ARN Ribosómico 16S / Secuencia de Bases / Reacción en Cadena de la Polimerasa / Incidencia / Análisis de Secuencia de ADN Tipo de estudio: Estudio diagnóstico / Estudio de incidencia / Estudio pronóstico Límite: Animales / Humanos País/Región como asunto: Asia Idioma: Coreano Revista: Korean Journal of Medicine Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Brucella / Brucella abortus / Brucelosis / Brucelosis Bovina / ADN / ARN Ribosómico 16S / Secuencia de Bases / Reacción en Cadena de la Polimerasa / Incidencia / Análisis de Secuencia de ADN Tipo de estudio: Estudio diagnóstico / Estudio de incidencia / Estudio pronóstico Límite: Animales / Humanos País/Región como asunto: Asia Idioma: Coreano Revista: Korean Journal of Medicine Año: 2006 Tipo del documento: Artículo