Effects of Intracoronary Administration of Autologous Adipose Tissue-Derived Stem Cells on Acute Myocardial Infarction in a Porcine Model
Yonsei Medical Journal
;
: 1522-1529, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-177074
ABSTRACT
PURPOSE:
Adipose-derived stem cells (ADSCs) are known to be potentially effective in regeneration of damaged tissue. We aimed to assess the effectiveness of intracoronary administration of ADSCs in reducing the infarction area and improving function after acute transmural myocardial infarction (MI) in a porcine model. MATERIALS ANDMETHODS:
ADSCs were obtained from each pig's abdominal subcutaneous fat tissue by simple liposuction. After 3 passages of 14-days culture, 2 million ADSCs were injected into the coronary artery 30 min after acute transmural MI. At baseline and 4 weeks after the ADSC injection, 99mTc methoxyisobutylisonitrile-single photon emission computed tomography (MIBISPECT) was performed to evaluate the left ventricular volume, left ventricular ejection fraction (LVEF; %), and perfusion defects as well as the myocardial salvage (%) and salvage index. At 4 weeks, each pig was sacrificed, and the heart was extracted and dissected. Gross and microscopic analyses with specific immunohistochemistry staining were then performed.RESULTS:
Analysis showed improvement in the perfusion defect, but not in the LVEF in the ADSC group (n=14), compared with the control group (n=14) (perfusion defect, -13.0+/-10.0 vs. -2.6+/-12.0, p=0.019; LVEF, -8.0+/-15.4 vs. -15.9+/-14.8, p=0.181). There was a tendency of reducing left ventricular volume in ADSC group. The ADSCs identified by stromal cell-derived factor-1 (SDF-1) staining were well co-localized by von Willebrand factor and Troponin T staining.CONCLUSION:
Intracoronary injection of cultured ADSCs improved myocardial perfusion in this porcine acute transmural MI model.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Células de la Médula Ósea
/
Tomografía Computarizada de Emisión de Fotón Único
/
Tejido Adiposo
/
Función Ventricular Izquierda
/
Tecnecio Tc 99m Sestamibi
/
Vasos Coronarios
/
Troponina T
/
Trasplante de Células Madre
/
Quimiocina CXCL12
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2015
Tipo del documento:
Artículo
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