Effect of Zoledronate on the Expression of Vascular Endothelial Growth Factor-A by Articular Chondrocytes and Synovial Cells: An in Vitro Study
Journal of Bone Metabolism
;
: 249-255, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-177279
ABSTRACT
BACKGROUND:
The aim of this in vitro study was to determine the effect of zoledronate, which is frequently used to treat osteoporosis, on osteoarthritis by analyzing zoledronate-induced expression of vascular endothelial growth factor-A (VEGF-A) in chondrocytes and synovial cells.METHODS:
After chondrocytes and synovial cells were separated and cultured, zoledronate was added, and VEGF-A and pigment epithelium-derived factor (PEDF) expression were quantified by real-time polymerase chain reaction and Western blotting.RESULTS:
There was no significant difference in the expression of VEGF-A mRNA in chondrocytes between the zoledronate group and the control group on the 8th day of culture. The expression of both VEGF-A and PEDF mRNA in synovial cells was significantly decreased in the zoledronate group (P<0.05).CONCLUSIONS:
Zoledronate decreases the expression of VEGF-A in synovial cells and may affect the development and progression of osteoarthritis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoartritis
/
Osteoporosis
/
ARN Mensajero
/
Western Blotting
/
Condrocitos
/
Factor A de Crecimiento Endotelial Vascular
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Idioma:
Inglés
Revista:
Journal of Bone Metabolism
Año:
2014
Tipo del documento:
Artículo
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