siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens
Genomics & Informatics
; : 55-57, 2013.
Article
en En
| WPRIM
| ID: wpr-177963
Biblioteca responsable:
WPRO
ABSTRACT
Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets. As multiple sources of variations that are unique to siRNA screens exist, there is a growing demand for a computational tool that generates normalized values and standardized scores. However, only a few tools have been available so far with limited usability. Here, we present siMacro, a fast and easy-to-use Microsoft Office Excel-based tool with a graphic user interface, designed to process single-condition or two-condition synthetic screen datasets. siMacro normalizes position and batch effects, censors outlier samples, and calculates Z-scores and robust Z-scores, with a spreadsheet output of >120,000 samples in under 1 minute.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
ARN Interferente Pequeño
/
Interferencia de ARN
/
Ensayos Analíticos de Alto Rendimiento
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Genomics & Informatics
Año:
2013
Tipo del documento:
Article